The N-Terminal Region of Severe Acute Respiratory Syndrome Coronavirus Protein 6 Induces Membrane Rearrangement and Enhances Virus Replication
Identifieur interne : 002914 ( Main/Exploration ); précédent : 002913; suivant : 002915The N-Terminal Region of Severe Acute Respiratory Syndrome Coronavirus Protein 6 Induces Membrane Rearrangement and Enhances Virus Replication
Auteurs : HAIXIA ZHOU [États-Unis] ; Debra Ferraro [États-Unis] ; JINCUN ZHAO [États-Unis] ; Snawar Hussain [États-Unis] ; JIANQIANG SHAO [États-Unis] ; Jonathan Trujillo [États-Unis] ; Jason Netland [États-Unis] ; Thomas Gallagher [États-Unis] ; Stanley Perlman [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2010.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) accessory protein 6 (p6) is a 63-amino-acid multifunctional Golgi-endoplasmic reticulum (ER) membrane-associated protein, with roles in enhancing virus replication and in evading the innate immune response to infection by inhibiting STAT1 (signal transducer and activator of transcription factor 1) translocation to the nucleus. Here, we demonstrate that p6 has an N-terminal region-cytoplasm-C-terminal region-cytoplasm configuration with residues 2 to 37 likely membrane embedded. Expression of p6, or of its N-terminal 41-amino-acid region, in the absence of other viral proteins, induced the formation of membranous structures, some of which were similar to double membrane vesicles involved in virus replication. Consistent with a role in virus replication, p6 partially colocalized with nonstructural protein 3 (nsp3), a marker for virus replication complexes. Further, while the C-terminal region is required for preventing STAT1 translocation to the nucleus, our results also indicated that the N-terminal 18 amino acids were necessary for maximal inhibition. Collectively, these results support the notion that p6 is a two-domain protein, although the function of each is not completely independent of the other.
Affiliations:
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Replication</title><author><name sortKey="Haixia Zhou" sort="Haixia Zhou" uniqKey="Haixia Zhou" last="Haixia Zhou">HAIXIA ZHOU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Iowa</s1><s2>Iowa City, Iowa</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Ferraro, Debra" sort="Ferraro, Debra" uniqKey="Ferraro D" first="Debra" last="Ferraro">Debra Ferraro</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Iowa</s1><s2>Iowa City, Iowa</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Jincun Zhao" sort="Jincun Zhao" uniqKey="Jincun Zhao" last="Jincun Zhao">JINCUN ZHAO</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Iowa</s1><s2>Iowa City, Iowa</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Hussain, Snawar" sort="Hussain, Snawar" uniqKey="Hussain S" first="Snawar" last="Hussain">Snawar Hussain</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Department of Microbiology and Immunology, Loyola University School of Medicine</s1><s2>Maywood, Illinois</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Jianqiang Shao" sort="Jianqiang Shao" uniqKey="Jianqiang Shao" last="Jianqiang Shao">JIANQIANG SHAO</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Central Microscopy Facility, University of Iowa</s1><s2>Iowa City, Iowa</s2><s3>USA</s3><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Trujillo, Jonathan" sort="Trujillo, Jonathan" uniqKey="Trujillo J" first="Jonathan" last="Trujillo">Jonathan Trujillo</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Interdisciplinary Program in Immunology, University of Iowa</s1><s2>Iowa City, Iowa</s2><s3>USA</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Interdisciplinary Program in Immunology, University of Iowa</s1><s2>Iowa City, Iowa</s2><s3>USA</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author><author><name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name><affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Department of Microbiology and Immunology, Loyola University School of Medicine</s1><s2>Maywood, Illinois</s2><s3>USA</s3><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Microbiology, University of Iowa</s1><s2>Iowa City, Iowa</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Iowa</region><settlement type="city">Iowa City</settlement></placeName><orgName type="university">Université de l'Iowa</orgName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>Membrane protein</term><term>Replication</term><term>Severe acute respiratory syndrome</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Protéine membranaire</term><term>Réplication</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) accessory protein 6 (p6) is a 63-amino-acid multifunctional Golgi-endoplasmic reticulum (ER) membrane-associated protein, with roles in enhancing virus replication and in evading the innate immune response to infection by inhibiting STAT1 (signal transducer and activator of transcription factor 1) translocation to the nucleus. Here, we demonstrate that p6 has an N-terminal region-cytoplasm-C-terminal region-cytoplasm configuration with residues 2 to 37 likely membrane embedded. Expression of p6, or of its N-terminal 41-amino-acid region, in the absence of other viral proteins, induced the formation of membranous structures, some of which were similar to double membrane vesicles involved in virus replication. Consistent with a role in virus replication, p6 partially colocalized with nonstructural protein 3 (nsp3), a marker for virus replication complexes. Further, while the C-terminal region is required for preventing STAT1 translocation to the nucleus, our results also indicated that the N-terminal 18 amino acids were necessary for maximal inhibition. Collectively, these results support the notion that p6 is a two-domain protein, although the function of each is not completely independent of the other.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Illinois</li><li>Iowa</li></region><settlement><li>Iowa City</li></settlement><orgName><li>Université de l'Iowa</li></orgName></list><tree><country name="États-Unis"><region name="Iowa"><name sortKey="Haixia Zhou" sort="Haixia Zhou" uniqKey="Haixia Zhou" last="Haixia Zhou">HAIXIA ZHOU</name></region><name sortKey="Ferraro, Debra" sort="Ferraro, Debra" uniqKey="Ferraro D" first="Debra" last="Ferraro">Debra Ferraro</name><name sortKey="Gallagher, Thomas" sort="Gallagher, Thomas" uniqKey="Gallagher T" first="Thomas" last="Gallagher">Thomas Gallagher</name><name sortKey="Hussain, Snawar" sort="Hussain, Snawar" uniqKey="Hussain S" first="Snawar" last="Hussain">Snawar Hussain</name><name sortKey="Jianqiang Shao" sort="Jianqiang Shao" uniqKey="Jianqiang Shao" last="Jianqiang Shao">JIANQIANG SHAO</name><name sortKey="Jincun Zhao" sort="Jincun Zhao" uniqKey="Jincun Zhao" last="Jincun Zhao">JINCUN ZHAO</name><name sortKey="Netland, Jason" sort="Netland, Jason" uniqKey="Netland J" first="Jason" last="Netland">Jason Netland</name><name sortKey="Perlman, Stanley" sort="Perlman, Stanley" uniqKey="Perlman S" first="Stanley" last="Perlman">Stanley Perlman</name><name sortKey="Trujillo, Jonathan" sort="Trujillo, Jonathan" uniqKey="Trujillo J" first="Jonathan" last="Trujillo">Jonathan Trujillo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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